The current study was carried out to investigate the effect of a Brain-based Learning Strategy on Developing The Reading Comprehension Skills of Faculty of Specific Education  Prospective Teachers . The study adopted the two groups design, in which 60 students participated from the fourth year, Instructional technology department at the Faculty of Specific Education, Zagazig University during the academic year 2023/2024. They were instructed by applying the brain-based learning strategy. To obtain data, one instrument was used: a pre-post reading comprehension skills test. Findings showed that the brain-based learning strategy improved students' reading comprehension skills. Thus, it is recommended to utilize brain-based learning as a new strategy in developing EFL students' reading comprehension skills.