This study was conducted to examine the reduction of chemical fertilizers in greenhouse sweet pepper production at the New Valley University College of Agriculture farm during the 2021/2022 and 2022/2023 seasons, namely cattle, poultry manure and compost and mineral fertilizers as control treatments .The study was conducted with the addition of these treatments. The results indicated the following characteristics and Yield ingredient of sweet peppers were greatly affected by fertilizer type. Highest values for both growth traits and yield ingredients were recorded when poultry manure was applied at 100% of the recommended fertilizer ,i.e., plant height, leaves number, branches number ,number of fruits/plant, fruit weight, fruit diameter, dry matter, fruit yield /plant, total water soluble phenols, carotenoids ,total flavonoids and antioxidant activities) both seasons were compared with mineral fertilizers (control). On the other hand, chlorophyll a, b and total carotenoids N, P And K content inside pepper leaves increased significantly in both seasons with the application of  poultry manure 25% of recommended fertilizer + 75% of mineral fertilizer vs. mineral fertilizer (control), while vitamin C, vitamin E and total fiber in pepper fruits increased significantly in both seasons .The application of 50% + 50% mineral fertilizer increased significantly compared to mineral fertilizer (control). It can be concluded that 100% application of  poultry manure as the recommended fertilizer resulted in improved growth, yield and quality of pepper.