Salt tolerant calli were obtained from anther culture of nine genotypes of bread wheat and their 36 F1 crosses to estimate combining ability effects for plant regeneration and green plantlet regeneration under different salt stress concentrations (control, 4, 8 and 12 g/L NaCl). The analysis of variance showed that salt stress levels mean squares were found to be highly significant for all in vitro studied traits. Considerable genetic variation among studied genotypes was observed for all in vitro studied traits under the four salinity stress treatments except albino plantlet regeneration at the two stress levels of 8 and 12 g/L NaCl. The highest frequencies of plant regeneration and green plantlet regeneration under the four salinity stress treatments were recorded for the two crosses P1 × P3 and P3 × P9, while the lowest ones were obtained in the two varieties; Giza-171 (P4) and Sids-14 (P6). High ratio of GCA/SCA was found more than unity for all in vitro studied traits under the four stress levels indicating that predominant role of additive gene action in the inheritance of all in vitro studied traits. Results showed that the parents   Gemmeiza-7 (P1), Gemmeiza-11 (P3) and Line-Azhar-1 (P9) were the best GCA for green plant regeneration under the four salt stress levels. The five crosses (P1 × P3, P1 × P7, P3 × P9, P4 × P8 and P5 × P6) appeared the best SCA effects for plant regeneration and regenerated green plantlet under salinity stress levels.