This work aimed to improve grapes productivity as well as chemical and physical properties of the berries using genetically modified bacteria as bio-nematicides against Meloidogyne incognita and as a bio-fertilizer by facilitating the solubilization of natural phosphate rocks and feldspar (as a potassium source), to achieve that, protoplast fusion experiment was performed between Peanibacillus polymyxa and Lysinibacillus sphaericus. Fourteen fusants were isolated, fusants F11 and F15 achieved 100% mortality in vitro. A field experiment was then conducted in Wady Elnatroon, El-Beheira governorate, Egypt, to evaluate the genetically modified bacteria as bio-nematicides and bio-fertilizer for Flame seedless in 2022 and 2023 at two doses of 100 and 200 mL/vine which were referred to as (A and B), and natural rocks at two rates (¼ kg rock phosphate + ½ kg feldspar) and (½ kg rock phosphate + 1 kg feldspar) were referred to as (1 and 2) The treatment F11B2 achieved the best decrease in M. incognita juveniles (J2) number, galls, and egg-masses compared to the control. Generally, all treatments improved the yield, physical and chemical characteristics of the berries, and leaf mineral content compared to untreated trees. The F11A1 increased all growth parameters, yield, quality, total phenols, and anthocyanins in berries, While, F11B2 recorded the best-improved nitrogen, phosphate, and potssium in both seasons.