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347363

The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Pharmaceutical Chemistry

Abstract

The study targets anti-hypothyroditic effect and phytochemistry of the Ficus carica L. leaves pet. ether extract. GC/MS data of the unsaponifiable fraction led to identification of 23 compounds (85.14%) while identified saponifiable fraction amounted 78.56%. TLC chromatography led to isolation of five triterpenes (α-amyrin, taraxerol, lupeol, 3, 22 dihydroxy-13 (18) oleanene and β- sitosterol). The extract exhibited promising in vitro anti-inflammatory response via fluorometric assay. The extract was orally given at a dose (500 mg/kg) where different biomarkers were measured in vivo: serum TSH, T3, T4, IL-6 and oxidative stress levels. Ficus carica pet. ether extract recorded amelioration in all parameters suggesting its significance against thyroid hypothyroidism induced in male rats as prophylaxis and treatment. Histological examinations of all the groups supported the results. Additionally, molecular docking studies reinforced the results, demonstrating that the major compounds exhibited high binding interactions with (COX-1), (5-LOX), (IL-6), and thyroid stimulating hormone receptor.

DOI

10.21608/ejchem.2024.273155.9393

Keywords

Anti-hypothyroidism, Ficus carica, GC/MS, Triterpenes, TSH, IL-6, Molecular docking

Authors

First Name

Marwa

Last Name

Elbatanony

MiddleName

M.

Affiliation

Pharmacognosy Department, National Research Centre (NRC), El Behouth St., P.O. 12622, Cairo, Egypt.

Email

batanony2016@gmail.com

City

-

Orcid

0000-0002-0483-7874

First Name

Hanan

Last Name

Aly

MiddleName

F.

Affiliation

Therapeutic Chemistry Department, National Research Centre (NRC), 33 El Buhouth St., P.O. 12622, Cairo, Egypt.

Email

hanan_abduallah@yahoo.com

City

-

Orcid

0000-0003-3642-1571

First Name

Doaa

Last Name

Deabes

MiddleName

Abd Elhady

Affiliation

Pharmacognosy Department, National Research Centre (NRC), El Behouth St., P.O. 12622, Cairo, Egypt.

Email

doaahady22@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

El-Sayed

MiddleName

F.

Affiliation

Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt., Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt.

Email

ahmedfikry.nrc@gmail.com

City

Giza

Orcid

0000-0002-5312-4870

First Name

Eman

Last Name

Younis

MiddleName

A.

Affiliation

Therapeutic Chemistry Department, National Research Centre (NRC), 33 El Buhouth St., P.O. 12622, Cairo, Egypt.

Email

youniseman530@yahoo.com

City

Cairo

Orcid

0000-0001-5195-6959

Volume

67

Article Issue

10

Related Issue

49535

Issue Date

2024-10-01

Receive Date

2024-02-27

Publish Date

2024-10-01

Page Start

341

Page End

359

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 67_Issue 10_Pages 341-359.pdf

PDF . 1.6MB

Link

https://ejchem.journals.ekb.eg/article_347363.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=347363

Order

347,363

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

The Anti-hypothyroidism Regulating Role of Ficus carica L. Leaves Extract: In-vitro, In-vivo, and In-silico Studies

Details

Type

Article

Created At

30 Dec 2024