The current study was conducted to assess the antioxidant, antibacterial, and anticancer activities of ethanolic extracts of defatted olive and plum stones (seeds and woody shells) and determined total phenolic compounds (TPCs), total flavonoids compounds content (TFCs) of extracts colorimetrically and identified by High-Performance Liquid Chromatography. Olive seed extract gave the highest content of TPCs (0.276g/100g.DW), while the highest content of TFCs was in plum seed extract (0.191g/100g.DW). There was a direct relationship between the antioxidant activity (reducing power, and scavenging activity of DPPH radical) and the extracts content of TPCs. Plum seed ethanol extract (EE) was the most effective extract against Pseudomonas aeruginosa, and Escherichia coli, while olive seed extract gave the most antimicrobial activity against staphylococcus aureus bacteria. On the other hand, olive and plum stone (seed, and woody shell) extracts have anticancer effect towards the two cell lines (HT-29, and MCF-7).