Rosemary essential oil is one of the most popular essential oils and has many health benefits. Rosemary essential oil is becoming increasingly important for its useful antioxidant activities or as a source of fragrance in cosmetics and foods. The present study conducted to evaluate the efficiency of essential oil recovery, qualitative and quantitative chemical components in rosemary essential oil in Lam Dong province. Three extraction methods Steam distillation (SD), Microwave-assisted hydrodistillation (MAHD) and Hydrodistillation (HD) were used for essential oil isolation and determination by gas chromatography and mass spectrometry. Essential oil yield was obtained by HD (0.9ml/g), SD (0.73ml/g), MAHD (1.4ml/g) method. 35 compounds were identified with the typical main components α-Pinene (18.317%, 23.455%, 25.667%), Eucalyptol (20.269%, 15.24%, 16.924%), Verbenone (11.56%, 10.111%, 11.881%), Bornyl acetate (8.116%, 10.111%, 7.516%),… Comparison of three extraction techniques of essential oils gives a clear difference in content and active ingredients based on analytical results.