The utilization of probiotics in ruminant diets has attracted a growing interest in providing various health and productive benefits. Enterococcus faecium characterization, encapsulation and evaluation as a potential probiotic for ruminants was the main aim of the current study. The isolated lactic acid bacteria (LAB) strains in this study was identified using MALDI-TOF MS technology and 16S ribosomal rDNA sequencing. The ability of the isolated LAB for producing antimicrobial agents as well as its tolerance to heat, acidity and bile salt was examined. Biomass production for the most potent LAB strain using permeate basal media was conducted. For feeding purpose; the produced biomass was encapsulated using extrusion method by sodium alginate. The encapsulated biomass effects on rumen fermentation and feed degradation were evaluated using in vitro batch culture technique. The results indicated that the isolated strain which met the specifications of the ideal probiotic was Enterococcus faecium EGY_NRC3. The addition of encapsulated E. faecium EGY_NRC3 to the ruminant ration led to higher degradability for the fiber fractions, dry matter (DM) and organic matter (OM) with higher production of total gases (TGP) and short chain fatty acids (SCFA) in the rumen in vitro. In conclusion; production of LAB probiotic using milk permeate locally will leading to reduce the importation cost for probiotics diminish environmental pollution hazards.