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274038

Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Biochemistry

Abstract

Six fungal species (T 1-6) and twelve isolates from bacterial fish wastes (BFW (1-7) and white pickled shavings wastes WPSW (1-5), excluded from Egyptian leather industry were examined for its utilization as a source for industrial gelatin and collagen hydrolysate. The conversion of the native/pretreated WPSW was conducted through protease blends in simultaneous processes. All the conducted studies confirmed the superiority and efficiency of the bacterial isolates in 2-d- shaken cultures at 37 ℃ over all the tested fungal species under the proper conditions for proteases, especially collagenases productivity. The three bacterial isolates BFW5, WPSW3 and WPSW5 proved their prevalence as potent collagenase producers, which stimulates good gelatin formation through the controlled enzymatic hydrolysis of the two WPS (native/pretreated) specimens, while at the same conditions, the isolates BFW7, WPSW3 & WPSW5 identified as (Citrobacter freundii) were nominated as potent hydrolysate producers. BFW5 identified as (Bacilus cirus) crude enzyme mixture surpassed all the others and afforded 42.5% lab scale gelatin formation efficiency, while the recorded hydrolysate % was only 5.97%. On the other hand, the bacterial isolate WPSW5 afforded the highest hydrolysate estimate (7.12%).

DOI

10.21608/ejchem.2022.160832.7007

Keywords

Enzymatic degradation, white pickled bovine pickled, industrial gelatin, Collagen Hydrolysate

Authors

First Name

abdelmohsen

Last Name

Saber

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Dept., Pharmaceutical and Drug Industries Research Div.National Research Centre, Dokki, Giza, Egypt P.O. Box: 12622

Email

abdelmohsen_ismail@yahoo.com

City

-

Orcid

0000-0001-6841-8866

First Name

Nabil

Last Name

Elsayed

MiddleName

hussein

Affiliation

NRC, cairo, Egypt

Email

nabil_17@yahoo.com

City

cairo

Orcid

0000-0003-2811-8855

First Name

Mostafa

Last Name

El-Abd

MiddleName

Abd El-Hameed

Affiliation

Chemistry of Natural Products, NRC, Giza, Egypt

Email

mostafaelabd77@yahoo.ca

City

-

Orcid

0000-0002-4604-7678

First Name

Amira

Last Name

Hassan

MiddleName

Aly

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical Industries Research Institute, National Research Centre, Dokki, Giza, Egypt.

Email

amiraalyhassan@yahoo.com

City

-

Orcid

0000-0002-4604-7678

First Name

Mohamed

Last Name

Abo Alkasem

MiddleName

Ibrahim

Affiliation

Chemistry of natural and microbial products, Pharmaceutical research division. National Research Centre, Giza, Egypt

Email

alkasem88@yahoo.com

City

-

Orcid

0000-0002-0077-6621

First Name

mohammed

Last Name

marzouk

MiddleName

sonhi

Affiliation

chemistry of tanning materials, chemical industry division. NRC

Email

msmarzouk@yahoo.co.uk

City

cairo

Orcid

0000-0002-1344-0617

First Name

hamed

Last Name

Elsayed

MiddleName

-

Affiliation

Chemistry of tanning materials and leather technology department, National Research center

Email

hamed_sci@yahoo.com

City

-

Orcid

0000-0002-6463-258X

Volume

66

Article Issue

3

Related Issue

40217

Issue Date

2023-03-01

Receive Date

2022-09-20

Publish Date

2023-03-01

Page Start

59

Page End

67

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_274038.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=274038

Order

274,038

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Enzymatic degradation of white bovine pickled shavings yielding industrial gelatin and collagen hydrolysate

Details

Type

Article

Created At

30 Dec 2024