Surgical sutures since ancient times have been the best solution for treating wounds. The risks associated with and limitations of wound closure devices demanded the need for cost-effective techniques and efficiency for wound healing. Until now, sutures and staples have been the main used tools in the biomedical industry. absorbable sutures which are based on biopolymer much preferred to nonabsorbable ones. absorbable sutures are degraded within the body usually by the aid of proteolytic enzymes or with hydrolysis. Although they are biocompatible, limitations in the lack of sufficient antimicrobials, and drug delivery which are desired for biomedical applications. This study aimed to modify the absorbable polyvinyl alcohol and chitosan a biopolymer mixed with GO as a nanomaterial and keratin as source of protein (PVA /Cs/GO/ Ke) sutures to support an antimicrobial effect that is protected from multidrug-resistant microorganisms. In addition, graphene oxide promised material for sutures enhancement. This could be because GO provides active chemical sites. GO has also been used to coat surgical sutures to improve their functionalities to prevent bacterial adherence. So, it's a good antimicrobial activity and drug delivery. Chitosan is a qualified antimicrobial agent can be attributed to its cationic nature and reduced water content. the mixture was turned to nanofiber by an electro-spun process. Then the fiber was turned to multifilament by a twisting machine The results advantages; first, absorbed nanofiber sutures with strong antimicrobial against pathogenic strains second, absorbed time controlled third cheapest biomaterial last but not least ecofriendly depends on was human hair.