This study has been proposed to study the effect of psyllium and chia seeds on obese rats. The chemical composition, phenolic compounds, amino acids, and fatty acids of psyllium and chia seed powders were determined. Thirty male albino rats were fed a standard diet for 7 days as an adaptation period, and then 24  rats were divided into two groups. The first group of six rats  was fed a basal diet for another 10 weeks and was considered the negative control group (G1). The second group was divided into four subgroups of six rats each. The first of the four subgroups was fed a high-fat diet (HFD) and was considered a positive control (G2). Other subgroups were fed on different diets for 10 weeks as they were fed on the  HFD supplement with psyllium and chia powder (G3, G4, and G5).The results of the biological experiment showed that adding psyllium and chia powder (5%) to the basal diet for obese rats, led to improved  liver and kidney functions, an increase in HDL-cholesterol, and a decrease in weight, blood glucose, TC, TG, LDL-cholesterol, and v-LDL cholesterol compared to the positive  control group (G2) . Through the results of this study, it was recommended   the possibility of using psyllium  and chia seeds be explored to improve  and reduce the symptoms caused by obesity.