This literature review provides an in-depth analysis of Bio-cement as an Eco-friendly substitute for conventional cement. Bio-cement is produced using microorganisms that produce calcium carbonate (CaCO3), which reduces the carbon footprint of cement production. The review covers the different types of microorganisms used, optimal conditions required for calcium carbonate production, the process of CaCO3 precipitation, and various test results. Additionally, the paper investigates the potential applications of Bio-cement in the construction industry, as well as the challenges and limitations associated with its use. The review highlights several studies on the use of different microorganisms, such as Sporosarcina pasteurii, Bacillus sp. MCP11, and Bacillus sp. CR2, to create Bio-cement. The mechanical properties of the final product are also analyzed, including compressive strength, tensile strength, and flexural strength. The paper provides valuable insights into the potential of Bio-cement as a sustainable alternative to conventional cement, as well as the potential limitations and challenges that need to be overcome to fully realize its benefits. Overall, this literature review is an essential resource for researchers and individuals interested in the sustainable construction industry. It provides a comprehensive analysis of the different aspects of Bio-cement, including its production process, properties, and potential applications. The review serves as a starting point for further research and development of Bio-cement as a viable and Eco-friendly alternative to conventional cement.