Postharvest diseases cause considerable losses to harvested date fruits during transportation and storage. The recent trend is shifting toward safer and more eco-friendly alternatives for the control of postharvest decays. Of various biological approaches, the use of antagonistic microorganisms is becoming popular throughout the world. Several postharvest diseases can now be controlled by microbial antagonists. In this study Trichoderma harzianum (1), T.asperellum, Trichoderma longibrachiatum, T.album, Trichoderma harzianum (2), and T.34 (T.asperellum) revealed different degrees of hyper parasitism to the growth of A.alternata, C.herbarum and Th.paradoxa on PDA medium. The greatest reduction occurring for C.herbarum, followed by A.alternata, while Th.paradoxa was less affected ones. Bacterial bioagents tested had inhibitory effect on the mycelial growth of the pathogenic fungi A.alternata, C.herbarum and Th.paradoxa compared with control. In this case, Brevandimonas vesicularis and Paenibacillus polymyxa gave the highest reduction of the mycelial growth of A.alternata, B.subtilis (1) and Ps.fiuorescens (1) came in the last.  In addition, Different concentrations of four bio-fungicides and bacteriocides were evaluated for their effectiveness to control date fruit rots under cold storage during 2020 and 2021 seasons. Futhermore, using (bio-control) followed by plant guard and Bio-Arc at 3g/L.W were the most effective biocides which minimized date fruit rot caused with A.alternata to a large degree compared with untreated date fruits. Generally, all tested fungicides and bio-agents were effective against the causal fungus in vitro and in vivo trials, but with different degrees.