The objective of this study was to evaluate the effect of N-fertilizer levels and vinasses on the yield and quality traits of two sugarcane varieties. Two field experiments were conducted at Shandaweel Agricultural Research Station in Sohag Governorate, Egypt in the 2020/2021 and 2021/2022 seasons to find out the appropriate level of nitrogen fertilizer (180, 210 and 240 kg N/fed.) and cane vinasses (0 and 50 l/fed.) to maximize yield and quality in sugarcane. Experiments were conducted using RCBD in a split split-plot arrangement. The results showed that sugarcane varieties differed markedly in all studied traits. The sugarcane variety G.T.54-9 recorded the highest values of yield and its component, while G.2003-47 was superior in juice quality traits in both seasons, as well as sugar yield/fed. in the 1^st one. Increasing N fertilizer level to 240 kg N/fed. resulted in a significant increase in yield and its component, in both seasons, while adding 210 kg N/fed. led to a significant increase in juice quality traits, in both seasons, as well as sugar yield/fed. in the 1^st one. Results showed that the addition of 50 l/fed. of vinasses caused a significant increase in all the studied traits in both seasons. In the present study, it was found that growing sugarcane varieties studied and fertilizing them with 240 and/or 210 kg N/fed., combined with 50 l/fed. of cane vinasses can be recommended to get the maximum cane and sugar yields/fed.