Fusarium sp is representing the one of the most important pathogens in causing a greet losses in tomato crops. Molecular identification show that the causal agent for tomato wilts in Minya governorate is F. oxysporum. Salmiya 65010 and Habiba are the most resistant genotype among the tested ones. T. harzianum and Pseudomonas fluorescence has the greatest inhibitory effect towards F. oxysporum growth. On the other hands, culture filtrates of T. harzianum and B. subtilus have the greatest inhibitory value on the pathogen growth (linear growth and dry weight). Also, T. harzianum significantly reduce the disease incidence and severity compared to control plants. It also shows some stimulation on growth parameter of tomato plants such as the plants height (cm), shoot length and root length, fresh weight of plants and dry weight of plant compare with the control.