Sugar beet plants are attacked by several diseases. The most serious diseases that causing post-harvest decay of sugar beet storage roots. Root rot affect sugar beet yield and quality. Various fungi and bacteria are reacted as root rotting incidents. Isolation trails resulted 8 infective bacterial isolates. Three bacterial isolates (B5, B6, B10) were the most virulent as root rotting. Morphological and biochemical properties of the most virulent bacterial isolates showed that they are typically reacted as Paenibacillus polymyxa. In addition, molecular identification of the most virulent isolate B5 confirmed it ' s identification as P. polymyxa. The obtained isolate B5 was rotted potato tuber slices. Response of sugar beet genotypes was various to P. polymyxa Infection. Sugar beet genotype BTS185 reacted as the most susceptible, while genotype Husam was the least susceptible to P. polymyxa infection. Furthermore, the tested isolate of P. polymyxa was able to infect storage roots of turnip, carrot and sweet potato beside garlic claves.