This study aim evaluation response nine wheat cultivars to treat by with three strains of Azotobacter bacteria (Azotobacter wild type, histidineless and histidineless × threonineless) in two seasons 2020/2021 and 2021/2022. Results confirmed that, treated wheat cultivars by Azotobacter histidineless (M2) recorded the highest mean on growth characters: Plant height (104.05 and 106.40 cm), number of tillers/plant (9.43 and 9.74), spike length (11.11 and 11.10 cm), number of spikelets / spike (20.46 and 20.67), 1000 grain weight (56.41 and 56.81 g), biological yield (6.96 and 7.14 ton/fed) and harvest index (38.68 and 40.65%) in seasons 2020/2021 and 2021/2022, respectively. While Sakha 95 cultivar was recorded the highest all growth character means : plant height (106.30 and 107.33cm), number of tillers/plant (9.88 and 11.33) and spike length, but Misr 1 cultivars was recorded the lowest plant height (91.26 and 91.26 cm), number of tillers/plant (8.04 and 9.05) and spike length (9.01 and 9.05 cm), biological yield (7.48 and 6.84 ton/fed) and Harvest index (41.67 and 42.75%) in seasons 2020/2021 and 2021/2022, respectively. Finally, we can summarize the results to the treatment of Sakha 95 wheat cultivars by Azotobacter histidineless record the best yield of wheat.