This study was conducted through the 2020 and 2021 seasons on prime seedless grapevines grown under Minia Governorate conditions to investigate the effects of five different nutrients: Amino acids (methionine, tryptophane, cystine, cysteine, and lysine) at 0.05 or 0.1%, algae extract at 0.1 or 0.2%, turmeric extract at 0.1 or 0.2%, boric acid at 0.05%, and gibberellic acid (GA3) at 20 ppm on vine vegetative growth characteristics, nutritional status, shot berries, yield, and quality of berries. All the nutrient treated vines showed enhanced growth aspects, nutritional status, yield, and berry quality compared with the untreated controls. The promotion of vegetative growth, vine nutritional status, and some chemical characteristics of berries were related to the application of some amino acids, algae extract, Turmeric extract, boric acid, and (GA3) in descending order. As for the yield, cluster aspects, berry weight, and dimensions, as well as reducing shot berries of clusters were related to the application of some amino acids, algae extract, Turmeric extract, GA3, and boric acid in descending order. Conclusively: Treatment of prime seedless grapevines three times at growth start, just after three growth starts later with some amino acids at 0.1% is the best treatment yield and gave high fruit quality.