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299346

Evaluation of potential synergistic activity of antimicrobial combinations against Colistin resistant Acinetobacter baumannii

Article

Last updated: 28 Dec 2024

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Subjects

-

Tags

Antimicrobial agents

Abstract

Background: Acinetobacter baumannii (A. baumannii) is one of the most important nosocomial pathogen. With the introduction of colistin as a last resort in treatment of A. baumannii, resistant strains. Using combined antibiotics could increase the success of treatment and reduce resistance. Objectives: To assess potential in-vitro synergistic activity of colistin when combined with (vancomycin, teicoplanin, rifampicin, tigecycline, meropenem, amikacin, and ceftazidime) against colistin resistant A. baumannii (CRAB). Also, to screen the prevalence of plasmid mediated colistin resistance (mcr) genes (mcr-1 to mcr-5) in colistin resistant isolates. Methodology: A. baumannii strains were isolated from different microbial specimens. Antibiotic susceptibility testing was done for all isolates by disk diffusion method while broth micro dilution (BMD) was performed to determine colistin minimum inhibitory concentration (MIC). Genotypic detection of mcr genes was done using multiplex PCR. Checkerboard method was done to detect  potential synergistic activities between colistin and other tested antibiotics against CRAB. Results: A total of 94 A. baumannii strains were isolated from 373 different clinical samples. Colistin resistance was detected in 44/94 (46.8%) of isolated A. baumannii. Only 2 out of 44 CRAB (4.5%) carried mcr-1 gene and neither of other mcr genes could be detected. All isolated CRAB were resistant to 7 tested antibiotics by BMD. But when colistin was included in a checkerboard pattern, colistin-based combinations with vancomycin, meropenem, rifampicin, teicoplanin and ceftazidime showed synergy in 93.2%, 90.9%, 88.7%, 86.4% and 79.5% of isolated CRAB respectively. For both tigecyclin and amikacin more than 90% of CRAB showed indifference in combination with colistin. Conclusion: Further studies are needed to determine the ability of colistin based antimicrobial combinations as an alternative therapy to treat CRAB infections and confirm that synergy.

DOI

10.21608/ejmm.2023.299346

Keywords

A. baumannii, checkerboard, Colistin, resistance, Synergy

Authors

First Name

Amal

Last Name

Dawoud

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Menoufia University, Egypt

Email

amalmohamed549@yahoo.com

City

Menoufia

Orcid

-

First Name

Sara

Last Name

Saied

MiddleName

Ahmed

Affiliation

Department of Clinical Pathology, National Liver Institute, Menoufia University, Egypt

Email

sarasaied@ymail.com

City

Menoufia

Orcid

-

First Name

Radwa

Last Name

Salem

MiddleName

H.

Affiliation

Department of Clinical Microbiology and Immunology, National Liver Institute, Menoufia University, Egypt

Email

salem_radwa@yahoo.com

City

Menoufia

Orcid

-

Volume

32

Article Issue

3

Related Issue

41216

Issue Date

2023-07-01

Receive Date

2023-05-16

Publish Date

2023-07-01

Page Start

31

Page End

39

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 32_Issue 3_Pages 31-39.pdf

PDF . 707.1kB

Link

https://ejmm.journals.ekb.eg/article_299346.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=299346

Order

299,346

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Evaluation of potential synergistic activity of antimicrobial combinations against Colistin resistant Acinetobacter baumannii

Details

Type

Article

Created At

28 Dec 2024