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A NEW USAGE OF SOFT PLASTINATION TECHNIQUE TO PREPARE HISTOLOGICAL SLICES OF ONE HUMPED CAMEL KIDNEY (CAMELUS DROMEDARIUS)

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Anatomy and embryology

Abstract

The soft plastination, or Elnady technique is primarily used for preserving anatomical specimens by replacing water and fat in the tissues with polymer. It is a novel, low cost technique which is used to produce anatomical teaching- aids, and can be modified to prepare microscopic sections.

for histology and pathology education.

In this study, the soft plastination technique was used to identify the advantages and disadvantages of the technique. Tissue samples of plastinated kidneys were obtained for histological study.

De-plastination refers to the process of removing the plastination medium, which is typically a plastic resin,. Acetone is commonly used as a solvent in the de-plastination process after soft plastination. De-plastination is the reverse process of plastination, where the polymer is dissolved to reveal the preserved specimen


The soft plastination technique is primarily used for preserving anatomical specimens by replacing water and fat in the tissues with polymer, it is a novel technique which is applied as an anatomical teaching aid and can be modified to prepare thin tissue sections. A modified technique was used to transform the plastinated specimens into microscopic sections for histology and pathology education. In this study, the soft plastination technique was used to identify the advantages and disadvantages of the technique. Tissue samples of plastinated kidneys were obtained for histological study. De-plastination refers to the process of removing the plastinated medium, which is typically a plastic resin. Acetone is commonly used as a solvent in the de-plastination process. De-plastination is the reverse process of plastination, where the polymer is dissolved to reveal the preserved specimen. After de-plastination, the samples were embedded in a mold of paraffin wax. The wax was allowed to solidify into blocks. The block was then cut into thin sections using a microtome. The microtome settings were adjusted to obtain sections of the desired thickness (typically around 5-10 micrometers for histological studies). The histological sections were then mounted onto glass microscope slides. Appropriate staining techniques were applied, such as hematoxylin and eosin (H&E), to enhance cellular details and highlight specific structures within the kidney tissue. The plastinated sections were compared to de-plastinated and formalin-fixed specimens. The soft plastination technique was found to be a helpful method for the examination of the microscopic specimens.

DOI

10.21608/avmj.2024.284106.1245

Keywords

SOFT PLASTINATION, camel, kidney

Authors

First Name

ALAA

Last Name

SAWAD

MiddleName

A.

Affiliation

Department of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, Iraq

Email

alaasawad24@gmail.com

City

-

Orcid

-

First Name

SAFAA

Last Name

AL. MUDHAFER

MiddleName

A.

Affiliation

Basra Health Department, Ministry of Health, Basrah, Iraq

Email

-

City

-

Orcid

-

First Name

WAFAA

Last Name

ALI

MiddleName

ABDULKAREEM

Affiliation

Basra Health Department, Ministry of Health, Basrah, Iraq

Email

-

City

-

Orcid

-

Volume

70

Article Issue

182

Related Issue

48831

Issue Date

2024-07-01

Receive Date

2024-04-20

Publish Date

2024-07-01

Page Start

351

Page End

357

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_366496.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=366496

Order

26

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

A NEW USAGE OF SOFT PLASTINATION TECHNIQUE TO PREPARE HISTOLOGICAL SLICES OF ONE HUMPED CAMEL KIDNEY (CAMELUS DROMEDARIUS)

Details

Type

Article

Created At

28 Dec 2024