Although fish are a valuable source of animal protein for humans, their production is sometimes reduced by parasite diseases. The purpose of this study was to ascertain the frequency and importance of possible parasites that could infect freshwater fish in the Egyptian governorate of Sohag. Atotal of 150 samples [100 Nile tilapia (Oreochromis niloticus) and 50 catfish (Clarias gariepinus)] were chosen at random from various farms in the Sohag Governorate for this purpose. According to the parasitological analysis of the collected fish, Clarias gariepinus had the highest infection rate 64% (32/50) compared to Oreochromis niloticus 56% (56 / 100). Quadriacanthus spp. (50%), Contracecum spp. (50%), Trichodina spp. (56%), Myxobolus spp. (55%), Cryptosporidium spp. (50%), and encysted metacercaria [microscopic (55%), and macroscopic (35%)] were the parasites found in Nile tilapia fish. In contrast, the identified parasites in catfish included microscopic encysted metacercaria (60%) and Quadriacanthus spp. (54%), Trichodina spp. (64%), Myxobolus spp. (44%), Henneguya spp. (40%), and Cryptosporidium spp. (56%). It is evident from the current study that a significant frequency of numerous zoonotic parasites were found in the fish under examination. As a result, control measures in fish farms are required to prevent the spread of these parasites, which pose a risk to public health.