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363599

MOLECULAR CHARACTERIZATION AND PHYLOGENIC ANALYSIS OF CRYPTOSPORIDIUM SPECIES ISOLATED FROM CATTLE IN SULAYMANIYAH, IRAQ

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Internal veterinary medicine

Abstract

Diarrhea is globally reported to be a common and major disease in newborn calves. According to the World Health Organization, it can cause a massive economic loss in the cattle industry. Thirty preweaning calves were collected. Calveswere taken from four different places in Sulaimaniyah province (Kalar, Halbjai Shahid, Khurmal, and Sharazor) between September 2022 and March 2023. The animals were divided into 3 age groups: (5–30 days), (31–60 days), and (61–90 days). Rectal swabs were being collected directly from diarrheal calves into plastic specimens for PCR testing. A fragment of the SSU rRNA gene was amplified using the Taq DNA master mix (Addbio, Republic of Korea). Phylogenetic trees were constructed based on a partial fragment of the SSU rRNA gene. The results showed that the higher infection rate (25%) appeared in calves less than one month old, while the lower infection rate (10%) appeared in the two months old and (12%) in three months old calves. In addition, the two sequences (BA/1/2022 and BA/2/2022) were C. ryanae, while the other sequence (BA/3/2022) was identified as C. parvum. This study reported the occurrence of Cryptosporidium species for the first time in four different places in Sulaimaniyah province, northern Iraq, using molecular techniques.

DOI

10.21608/avmj.2024.270775.1228

Keywords

Cryptosporidium parvum, Cryptosporidium ryanae, diarrhea, Phylogenic analysis

Authors

First Name

BASIM

Last Name

ALI

MiddleName

ABDULWAHID

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region-Iraq.

Email

basim.ali@univsul.edu.iq

City

-

Orcid

-

First Name

HARDI

Last Name

MARIF

MiddleName

FATTAH

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani.

Email

hardi.marif@univsul.edu.iq

City

Sulaymaniyah

Orcid

0000-0002-8940-396X

First Name

KWESTAN

Last Name

ALI

MiddleName

NAJM

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani.

Email

kwestan.ali@univsul.edu.iq

City

Sulaymaniyah

Orcid

0000-0002-2171-0357

First Name

HANA

Last Name

RAOOF

MiddleName

SHERZAD

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaimaniyah, Iraq

Email

hana.raoof@univsul.edu.iq

City

-

Orcid

-

First Name

RIZGAR

Last Name

SULAIMAN

MiddleName

RAHIM

Affiliation

Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaimaniyah, Iraq

Email

rizgar.rahim@unisul.edu.iq

City

-

Orcid

-

First Name

MOHAMMED

Last Name

BABA SHEIKH

MiddleName

OMER

Affiliation

Directorate of Veterinary in Sulaimani, Salim Street, Sulaimaniyah, Kurdistan Region, Iraq

Email

mohammed.babasheikh@uhd.edu.iq

City

-

Orcid

-

Volume

70

Article Issue

182

Related Issue

48831

Issue Date

2024-07-01

Receive Date

2024-03-26

Publish Date

2024-07-01

Page Start

125

Page End

135

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 70_Issue 182_Pages 125-135.pdf

PDF . 842.6kB

Link

https://avmj.journals.ekb.eg/article_363599.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=363599

Order

11

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

MOLECULAR CHARACTERIZATION AND PHYLOGENIC ANALYSIS OF CRYPTOSPORIDIUM SPECIES ISOLATED FROM CATTLE IN SULAYMANIYAH, IRAQ

Details

Type

Article

Created At

28 Dec 2024