A cross-sectional study has been undertaken in Minya province to survey an outbreak of foot-and-mouth disease (FMD) in cattle and identify the associated risk factors. From November 2021 to October 2022, researchers collected 100 blood samples, ten tongue epithelium specimens, and saliva swabs from animals of various ages and sexes in different locations. Additionally, the animals were examined for FMD signs. The overall seroprevalence was tested via SNT, which was 67%. Tissue samples and swabs were isolated and examined using conventional RT-PCR for detection of the FMD serotype, revealing serotype A. Statistically, no significant variation (P > 0.05) was observed in the prevalence of FMD in relation to age, sex, season, or locality. The findings of this study indicated that the recent FMD outbreak has been caused by serotype A in the studied locations and that FMD is widespread in cattle populations in Minya province. These findings underline the necessity for immediate attention and future research to detect FMDV strains spreading in the region that aid in the execution of effective prevention strategies.