Ninty-four clinical samples were collected as swabs from cows suffered from different types of wounds in Baghdad governorate. Samples were cultured on different agar and biochemically tested for identify the type of bacteria that colonize in the wounds and to isolate and identify P. aeruginosa. The results revealed that out of 94 samples collected from wounds, 87 bacteria isolate (90.55%) were grow bacterial growth and consider positive samples, while negative growth appear to be 7.4%. The profile of bacteria in the cultured samples revealed bacteria as fellow : P. aeruginosa 36 (36.78%) was the most common isolate followed by S. aureus22(25.28%), E. coli 21(24.1%), K. pneumoniae 5(5.7%), P. mirabilis 3(3.4%).

Thirtysix P. aeruginosa isolates were tested for antibiotic sensitivity. All isolate (100.0%) are resistant to Ampicilin-Sulbactam, Ticracillin-pyostacine Ticracillin-,clavulnic acid. and Ticracillin, these isolates show high resistance to Cotrimoxazol 94.4%, Cefepim 77.7% , Ceftazidime 66.6%, Imipenem 63.8%, Meropenem 61.1% while show low resistance to Gentamicin 44.4%, Tobramycin 38.8%, Piperacillin-pyostacine 27.7%, Piperacillin- Tazobactam 30.5%, Piperacillin- Tazobactam-pyostacine 33.3%, Colistin 33.3%, Ciprofloxacin 22.2% and Amikacin 22.2%, and among these antibiotics, Amikacin and Ciprofloxacin was the most effective antibiotic against P. aeruginosa isolates.