Aquaculture is a main contributor to food and nutrition security, while Egypt is a leading producer. Gilt-head seabream (Sparus aurata) is a target for intensification as it is a valuable luxury food. Vibriosis is the most significant disease of maricultur representing a great challenge that could hamper the sustainability of the aquaculture industry. In this study, we aimed to evaluate the modulatory effect of combined probiotic "Lactobacillus fermentum and lactobacillus delbrueckii" on the experimentally-infected seabream by vibrio spp. One hundred and twenty seabreams were divided into four groups: the first two groups were fed non supplemented diet, while the 3rd and 4th groups were fed probiotic-supplemented and florfenicol-supplemented diets, respectively. Hematological, biochemical, growth and feed utilization parameters and pathological examination were applied to all experimental groups. Our result showed that the probiotic-supplemented diet was able to enhance the growth performance and feed utilization parameters, as well as the survivability of the experimentally-infected seabream. In addition, the heamatological, biochemical parameters and histopathological findings were also improved over the control values.