Campylobacter species are Gram-negative bacilli that are characterized by being catalase-positive, oxidase-positive, motile, microaerophilic bacteria, and non-spore-forming. Campylobacter is often isolated from animal sources, as it inhabits the gastrointestinal tract of both wild and domestic animals and birds, especially poultry. Milk may be possibly contaminated by the direct discharge of a mastitis-affected cow or by excrement from diseased or colonized cattle during milking. Powdered infant formula (PIF) is considered a non-sterile product and may be contaminated intrinsically or extrinsically with various bacteria that can cause critical illness in infants. This study was performed to detect and identify Campylobacter spp. in powdered infant milk formula by phenotypic and genotypic methods. Ten isolates of Campylobacter from eighty-six samples were phenotypically identified and confirmed genotypically by PCR, with a pattern of 11.6%. This Campylobacter could potentially be transmitted to children by PIF consumption that has not been adequately handled, prepared or processed.