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331778

BULL SPERM RHEOTAXIS AND KINEMATICS IN MICROFLUIDIC CHANNELS WITH DIFFERENT HEIGHTS

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

Artificial insemination, embryotransferand Assisted Reproductive techniques

Abstract

Recently, microfluidics has attracted a lot of attention in reproduction due to its ability to simulate the natural environment inside the female reproductive tract and its ability to separate high-quality sperm cells using rheotaxis as a selection mechanism. Positive rheotaxis refers to the ability of the sperm to direct itself and swim against the liquid current during the fertilization journey. In this study, we investigated the effects of various microchannel heights on sperm positive rheotaxis (PR) and sperm kinematics. We used two microchannels with the same width (200µm) and different heights (channel 1= 20 µm height and channel 2= 100 µm height). Sperm samples were obtained from two bulls with known fertility and analyzed using CASA. The results showed that PR and all sperm kinematics (VCL, VSL, VAP, and BCF), except linearity, were significantly higher in microchannel 2 with a greater height (100 µm) than in microchannel 1 with a lower height (20 µm). Our findings indicate that PR percentage and sperm kinematic values in microfluidic platforms depend mainly on microchannel dimensions, which have a direct influence on the velocity profile inside the channel. These results could be useful for the development of microfluidic devices used for sperm separation and selection in the future. 

DOI

10.21608/avmj.2023.228274.1176

Keywords

Microchannel dimension, sperm rheotaxis, cryopreserved semen, CASA parameters, cattle

Authors

First Name

HAITHAM

Last Name

MOFADEL

MiddleName

A.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.

Email

haitham_mofadel@aun.edu.eg

City

Assiut

Orcid

https://orcid.org/00

First Name

HASSAN

Last Name

HUSSIEN

MiddleName

A.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.

Email

hassan.hussien@vet.au.edu.eg

City

assiut

Orcid

-

First Name

AHMED

Last Name

FATH EL-BAB

MiddleName

M.R.

Affiliation

Mechatronics and Robotics Department, Egypt-Japan University of Science and Technology (E-JUST), Alexandria 21934, Egypt

Email

ahmed.rashad@ejust.edu.eg

City

Alexandria

Orcid

-

First Name

TAYMOUR

Last Name

EL‑SHERRY

MiddleName

M.

Affiliation

Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt

Email

timour.ibrahim@vet.au.edu.eg

City

Assiut

Orcid

-

Volume

70

Article Issue

180

Related Issue

45134

Issue Date

2024-01-01

Receive Date

2023-08-14

Publish Date

2024-01-01

Page Start

50

Page End

61

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 70_Issue 180_Pages 50-61.pdf

PDF . 1.3MB

Link

https://avmj.journals.ekb.eg/article_331778.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=331778

Order

5

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

BULL SPERM RHEOTAXIS AND KINEMATICS IN MICROFLUIDIC CHANNELS WITH DIFFERENT HEIGHTS

Details

Type

Article

Created At

28 Dec 2024