The present work was conducted to study the comparative among whey protein, amino acids and chia seedsinenhancing the performance of endurance sports in rats.The chemical composition and fatty acid content of chia seeds were analyzed. Forty-eight albino rats (Sprague Dawley Strain) (150±5g) were divided into four groups (12 of each). The first main group fed on basal diet for 28 days and served as -ve control group. Groups from 2-4 were fed on a basal diet containing 10% whey protein, amino acids and chia seeds, respectively for 28 days. All 4 groups had the same swimming protocol for 28 days. The chemical composition of chia seeds showed that the highest content was carbohydrates followed by fats and protein with values of 39.5, 30 and 21, respectively. Chia seeds fatty acid contains the highest value of linoleic acid followed by γ-linolenic acid. Feeding rats led to a significant increase in creatinine kinase, lactate dehydrogenase, serumalkaline phosphatase (ALP), ammonia, and glutathione-S-transferase (GST) in all tested groups compared withthe control group.
 
While there wasa significant decrease in serum glucose and Malondialdehyde in tested groups. There wasa significant decrease in serum aspartate aminotransferase (AST) in groups fed on whey protein and amino acids,while was no difference in group was fed on chia seeds. There was a significant decrease in creatinine and uric acid in the group that was fed on chia seeds, results recorded significant increase in serum alanine transaminase in the groups fed on amino acids and chia seeds, while it records a significant decrease in group fed on whey protein. The highest increase in serum creatinine recorded in the group was fed on amino acids. According to the above results chia seeds have a beneficial effect on enhancing the performance of endurance sports in rats which may be used with human beings.