The present work used ZnO-NPs from the aqueous extracts of Moringa Oleifera, Ocimum Tenuiflorum, and Neem (Azadirachta indica) leaves as adsorption for the removal of heavy metals such as Cu+2 and Ni+2 were studied to investigate the effect of zinc oxide nanoparticles ZnO-NPs versus contact time and different concentrations. The maximum percentages of Cu (II) and Ni (II) versus contact time removal were found to be (99.94%) from the Neem (Azadirachta indica) leaves and Ocimum Tenuiflorum leaves for Cu (II) and (75.70%) from Neem (Azadirachta indica) leaves for               Ni (II), respectively at the same time (50 min). In cases of versus the difference concentration percentages of Cu (II) and           Ni (II) of ZnO-NPs, the highest produced from Neem (Azadirachta indica) leaves was (99.99 and 97.60 respectively) at the same dose (0.01g/100 ml). Also, the ZnO-NPs exhibited strong antibacterial activity was evaluated toward were tested for Bacillus subtilis (gram-positive) and E. coli (gram-negative) bacteria at a concentration (75, 150, 300, 450, and 600 mg/ml), the highest produced at the concentration (600 mg/ml) the antibacterial activity towards varied extensively with the synthesis parameters. This study presents an efficient green synthesis of ZnO nanoparticles that have potential applications in antibacterial activity.