Finding novel active compounds to solve the problem of microbial resistance of antibiotic is a matter of urgency. That's why several drugs were produced from many precursors. Schiff bases are a good base for synthesis several antimicrobial compounds . This work investigates  biological activities as cytotoxicity and antimicrobial properties of some Schiff base compounds which synthesized by coupling reaction between two aromatic amines namely: aminoantipyrine, and 4-aminophenol by two aldehydes namely: vanillin and gluteraldehyde. Metal complexes of the obtained four Schiff bases were prepared throughout reaction with copper and ferric ions. FTIR, atomic absorption spectroscopy and  ¹H-NMR measurements. microbial evaluation of Schiff bases and metal complexes was tested against various microorganisms including: gram +ve and gram -ve  bacterial strains utilizing the diameter of inhibition zone, and minimum inhibitory concentration procedures. Showing that the cytotoxic effect of metal complexes is more than the ligand form . Furthermore, Ferric metal complexes exhibited significant inhibition than the copper complexes, which was attributed to the ability of oxidation-reduction tendency of Fe³⁺ ions than Cu²⁺. The gram genera of the bacterial strains played a significant role on their resistivity towards the tested antimicrobial compounds, own to the nature of their cellular membranes.