Stray cats are highly susceptible to a variety of parasitic infections because they have the habit of roaming freely. A total of 50 stray cats were collected from various areas in Alexandria governorate. The results indicated that 17 (34%) stray cats were infected with heterophyid species. The identified heterophyid species were Heterophyes heterophyes (16%) then Haplorchis taichue (8%), Pygidiopsis geneta (6%), Ascocotyle longa (4%), Stictodora sawakinsis (4%) and Procerovum varium (2%). DNA sequencing of 539 bp of ITS2 gene was generated. As shown in sequence distance figure, the sequenced strains showed 99.5 - 100% identity to H. heterophyes strains confirming the clustering of the study strain with H. heterophyes. The study revealed that stray cats are a reliable indicator of fish-borne heterophyid species in the environment given that their relevance of public health must be carefully considered.