The different symptoms of the herd were the motive to study the immunological state of the herd. Fifty sheep and eight goats of different ages from Herd with a history of recurrent abortions and suffering from abscesses, respiratory manifestation and diarrhea in El Fayoum Governorate, Egypt were investigated. 58 serum samples, 58 nasal swabs, 58 fecal swabs, 12 milk samples, 4 pus swabs, and 6 animal feeds samples were gathered to determine the bacteriological and fungal causative agents. The traditional culture methods for diagnosing Brucella melitiensis were confirmed by the polymerase chain reaction (PCR) test. Sensitivity test was applied to detect the effective antibiotic and antimycotic for each field strain isolate and to avoid microbial resistance.
Our research showed different prevalence of bacterial and fungal isolated from obtained samples from sheep and goat herd. Tested animals showed mixed infections with bacterial and mycotic pathogens represented 60.34%, bacteriological infection only was 5.17 % and mycotic infection only was 27.58% that reflected on immunity parameters and drugs sensitivity results.