Loop-mediated isothermal amplification (LAMP) is a powerful diagnostic tool with various applications that extend beyond clinical settings, including environmental monitoring and food quality control. It is characterized by its reliability, exceptional sensitivity, and specificity in amplifying target DNA, employing up to six primers. The energy-efficient and isothermal nature of LAMP enhances its appeal, making it a cost-effective option for point-of-care diagnostics. In this study, we investigated rapid detection methods for Staphylococcus aureus in dairy products, focusing on the Loop-mediated Isothermal Amplification (LAMP) technique. Our results demonstrate that LAMP is not only one of the fastest but also one of the most cost-effective methods available compared to polymerase chain reaction (PCR). Validation tests revealed that LAMP exhibits high sensitivity relative to PCR, with experimental data showing a strong correlation between the two techniques. Furthermore, we established that mecA-specific LAMP assays can be completed in under an hour while maintaining excellent specificity and sensitivity. These findings suggest that LAMP could be a viable alternative for quick and reliable detection of Staphylococcus aureus in dairy products, potentially enhancing food safety protocols.