Dairy cows play an important role in production in our country Egypt. Transition period is the most critical time for those cows that undergo different hormonal and biochemical changes. We take care of animals during this period by addition of mixture of vitamins and trace minerals to the ration in order to improve their general health. The present study was established for evaluation the effect of addition of some trace minerals and vitamin E to ration on some hormonal and biochemical parameters in dairy cows. We used 50 pregnant dairy cows for this study. We divided them into 5 groups. Group 1 (control), supplemented group 2 (vit E), group 3 (Cu sulphate), group 4 (Mg sulphate) and group 5 (Zn oxide). Samples were collected from animals at 2^nd, 4^th&6^th week before parturition and at 2^nd, 4^th& 6^th week after parturition. The result of the present study revealed an increase in the levels of glucose, GSH, SOD, Ca, P, Mg and decrease level of cortisol, MDA& haptoglobin in all supplemented groups when compared to control at different stage of pregnancy.