In old fashion poultry plant in Menoufia governorate, seventy five chicken carcasses were divided into 5 groups (15 for each) and packed into plastic bags after sprayed with water, potassium sorbate (2% and 2.5%) and natamycin (0.1% and 0.2%). They were kept in a refrigerator at 40 C. After a day, the samples were analyzed to determine how these treatments had affected their mycological profile. When natamycin (0.1% and 0.2%) was used, there was a greater reduction percentage in the total mold counts (91.1% and 97.2%). While, lower reduction percent in the total molds count by using of potassium sorbate (2% and 2.5%) were 69.4% and 82.6%, respectively. A. flavus (40%), A. fumigatus (6.7%), A. niger (13.3%), A. ochraceus (13.3%), and A. tereus (6.7%) were the Aspergillus species isolated from the control group; A. flavus (33.3%), A. fumigatus (6.7%), A. niger (13.3%), A. ochraceus (6.7%), and A. tereus (6.7%) were the species isolated from the potassium sorbate (2%) groups. A. flavus (20%), A. niger (13.3%), A.ochraceus (6.7%), and A. tereus (6.7%) were the isolation percentages in the potassium sorbate (2.5%) group, A. flavus (20%), A. niger (6.7%), A. ochraceus (6.7%) in the natamycin (0.1%) group while A. flavus (13.3%) and A. niger (6.7%) were isolated from the natamycin (0.2%) group. Toxigenic A. flavus isolated from control, potassium sorbate (2%), potassium sorbate (2.5%) and natamycin (0.1%) treated groups were 26.7%, 26.7%, 13.3% and 6.7%, respectively but there are no toxigenic strains of A. flavus isolated from 0.2% Natamycin treated group. Aflatoxins B1, B2, G1 and G2 were extracted from control and potassium sorbate (2%) groups but B1 and B2 were extracted from potassium sorbate (2.5%) group. While, in 0.1% Natamycin group B1 is the only aflatoxin that extracted. Generally, natamycin proved to be more efficient than potassium sorbate in suppression of mold growth in chicken carcasses and higher concentrations are better. So, the use of natamycin (0.2 %), as it is safe antifungal agent, is recommended to improve safety of chicken carcasses.