Fifty three pneumonic cattle lung of both sexes were sampled. Fourteen  bacterial isolates (12 P. multocida and 2 M. haemolytica) were positively approved from lung tissue. Isolation occurred by ordinary colonial and biochemical confirmation. cultural characteristics were identified on to blood agar from brain heart infusion broth by ordinary methods.  Isolates harbored omp87, nanB and toxA virulence genes (75%, 58.3% and 66.7%) for P. multocida, while lktC virulence genes (100%) were determined in all of tested M. haemolytica isolates. Histopathology of the examined lungs were performed and the microscopical changes were described. Immunohistochemical staining of the positive reactors reveal significant increase of CD68 cells in P. multocida and M. haemolytica affected tissues; Chronic interstitial pneumonia (P. multocida), Fibrinous pneumonia (P. multocida), chronic bronchopneumonia (P. multocida) and Chronic pneumonia (M.haemolutica) (2.66, 4.43,5.40 and 6.21) respectively. In summary, detection of virulence genes is important for more controllable method of the pathogen and CD68 cell IHC tool approved its ability pathologically for confirmatory diagnosis of inflammation and pathological alterations exhibited by P. multocida and M.haemolutica pathogens.