This study was planned to investigate the effect of milk fever and treatment on haemato-biochemical parameters alterations in buffaloes. About 15, 4-5 years old buffaloes (5 healthy -10 suffering from milk fever) were divided into 3 groups (5/each). 1^st group healthy buffaloes served as control, 2^nd group buffaloes suffering from slight milk fever or the first stage of milk fever (tremors in head and legs) and treated by 500 ml Calcium borogluconate (I/V) as one dose, along with 25 ml phosphonic acid/buffalo (I/V), while the 3^rd group buffaloes were suffering from severe milk fever (depression, lateral recumbancy beside unconsciousness)and  treated by 500 ml Calcium borogluconate (I/V) as one dose and  25 ml phosphonic acid(I/V)/buffaloe along with 1.5ml/50 kg bwt vitamin D (I/M) daily for 5 days. Blood samples were taken from each buffalo one pre and at 5^th days post treatment for determination of hematobiochemical parameters changes.
Buffaloes suffering from milk fever revealed a significant reduction in calcium, phosphorus, α, β globulins, superoxide dismutase (SOD), catalase (CAT), glutathione(GSH), glutathione peroxidase (GSH-px) beside an insignificant reduction in magnesium, potassium, red blood cells (RBCs), hemoglobin (Hb), packed cell volume (PCV), lymphocyte, basophil, monocyte, total protein, albumin , total globulin coupled with a significant increase in glucose, white blood cells (WBCs), neutrophil, eosinophil, cortisol, parathyroid, aspartate amino transferase ( AST), alanine amino transferase(ALT), alkaline phosphatase (ALP), creatinin kinase (CK), creatinin phosphkinase (CPK), lactate dehydrogenase (LDH),and malondialdehyde (MDA) associated with significant increase in γ globulin.
Treatment of diseased buffaloes using Calcium borogluconate, phosphonic acid and vitamin D improved its healthy status as well the hematobiochemical parameters and returned to nearly normal levels at 5^th day post treatment when compared with healthy control buffaloes.
Summaring up our observations, it could be concluded that milk fever in buffaloes induced some adverse effects in hematobiomcheical parameters and oxidation. Treatment of diseased buffaloes by Calcium borogluconate, phosphonic acid and vitamin D induced cure of diseased buffaloes and improved the adverse effects of milk fever.