Avian influenza virus (AIV) is one of the most important viral diseases that widely affect the Egyptian commercial poultry industry, with varying mortality rates and clinical symptoms. AIV is a catastrophic disease of poultry belonging to the family Orthomyxoviridae, genus Influenza A virus. This study aims to identify, analyze, and resolve issues related to the epidemiology, and diagnosis of currently prevalent AIV subtypes in Egyptian field. Molecular evidence of AIV subtypes was investigated in domesticated commercial poultry in three Egyptian provinces (Ismailia, Sharqia, and Dakahlia) between 2022 and 2023. 50 tissue samples were examined by Real-time reverse transcription polymerase chain reaction (RT-qPCR) followed by isolation trial and serological identification through hemagglutination (HA) and hemagglutination inhibition test (HI)  of the 9 positive samples (8 H9, and 1H5). The isolated samples were negative for AIV by HI test; this result may be due to several reasons as the presence of degraded nucleic acid, but not active virus or the samples collected during the convalescent stage. From this result, it can be concluded that the continuous circulating of H9 and H5 viruses detected in this study necessitates regular virus monitoring for better control. The objective of this study was molecular investigation of AIV in domestic in Egypt, focusing on H5, H9 and H7 subtypes.