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332459

Detection of Cryptosporidium parvum in locally manufactured yoghurt and Kareish cheese

Article

Last updated: 28 Dec 2024

Subjects

-

Tags

Food Safety

Abstract

Cryptosporidium parvum is an important zoonotic parasite causing severe diarrhea in humans and young animals. Fifty milk products (25 Baladi yoghurt and 25 Kareish cheese) were tested for detection of C. parvum oocysts. The prevalence of C. parvum in yoghurt was 44%, 36% and 12% by microscopic examination, experimental infection and PCR, respectively. There is a statistical significant difference of C. parvum in yoghurt between microscopic examination and PCR as well as between experimental infection and PCR. While, its prevalence in Kareish cheese was 24%, 44% and 20% by microscopic examination, experimental infection and PCR, respectively. No statistical significant difference of C. parvum were detected in Kareish cheese between microscopic examination, experimental infection and PCR. Microscopic examination of prepared yoghurt and Kareish cheese smears and experimentally infected rats fecal smears showed the spherical shape of C. parvum oocysts (4-6μm). A banana shape of C. parvum sporozoite (3.2x1.4μm) was found in fecal smear of experimentally infected rat by contaminated yoghurt. Meanwhile, the PCR identified the specific Actin gene of C. parvum in 3 yoghurt and 5 Kareish cheese samples. From the obtained results, it could be concluded that C. parvum oocysts remain viable in locally made milk products.  

DOI

10.21608/ejah.2024.332459

Keywords

Cryptosporidium parvum, microscopic milk products PCR

Volume

4

Article Issue

1

Related Issue

43863

Issue Date

2024-01-01

Receive Date

2023-10-19

Publish Date

2024-01-01

Page Start

164

Page End

175

Print ISSN

2735-4938

Online ISSN

2735-4946

Link

https://ejah.journals.ekb.eg/article_332459.html

Detail API

https://ejah.journals.ekb.eg/service?article_code=332459

Order

332,459

Type

Original researches

Type Code

1,636

Publication Type

Journal

Publication Title

Egyptian Journal of Animal Health

Publication Link

https://ejah.journals.ekb.eg/

MainTitle

Detection of Cryptosporidium parvum in locally manufactured yoghurt and Kareish cheese

Details

Type

Article

Created At

28 Dec 2024