Clostridium perfringens (C. perfringens) is the pathogenic bacterium caused necrotic enteritis (NE) which is the most threatening problem in poultry sector. This work aimed to study the occurrence of Clostridium perfringens, find out its in-vitro antibiotic sensitivity pattern to various antimicrobial drugs and the experimentally efficacy of antibiotic (amoxicillin) alone or including a probiotic against necrotic enteritis NE. The occurrence rate of C. perfringens in examined 150 tissue samples was 38.6%. Antibiogram analysis revealed that the organisms are very susceptible to amoxicillin (74.1%) followed by bacitracin (70.6%). Almost of isolates were multidrug resistant (MDR). Toxinotyping of C. perfringens isolates by PCR showed that all tested isolates belonged to C.perfringens type A. In the experiment, one day-old Cobb broiler (N=120) were divided into six equal-sized groups. Group (A) was provided with balanced ration without treatment (control negative), group (B) was fed on balanced ration and infected with C. perfringens (control positive), group (C) was infected with C. perfringens and treated with selected antibiotic (amoxicillin) in drinking water for 3 consecutive days by dose of 1gm/ L 24 hours post infection, group (D) was received probiotic in the drinking water from one day-old and then infected with C. perfringens, group (E) was received probiotic in the drinking water from one day-old and then infected with C. perfringens then treated with selected antibiotic (amoxicillin) by the same dose 24 hours post infection and group (F) was infected with C. perfringens and treated with selected antibiotic (amoxicillin) and probiotic by the same dose 24 hours post infection. The results showed that birds received probiotic displayed higher BW, BWG and most improved FCR over the whole rearing period. Chickens infected with C. perfringens had characteristic effect in hematological and biochemical parameters with significant decrease in total protein and HDL. The treated groups revealed significant improvement in above mentioned parameters. Experimentally infected chickens with C. perfringens developed atypically clinicopathological pictures of NE with 30% mortality. A milder disease picture has been recorded in treated groups with amoxicillin and or probiotic. According to our research, adding probiotic supplements to a chicken's diet can improve performance, gut health, and blood components to protect the bird against C. perfringens infection.