The present study was designed to investigate the effects of mixture Sodium calcium edetate (CaNa2EDTA) and Alpha lipoic acid (ALA) with chia seeds powder (ChS.P) against lead and cadmium-induced Neurotoxicity in rats. Twenty-four healthy Albino male rats, weighing 158 ± 3 g were subdivided randomly into four groups (6 each), one served as negative control group (-ve) and lead-cadmium induced neurotoxicity rats, that classified into untreated neurotoxicity group (+ve) and two treated groups with CaNa2EDTA and ALA combined and ChS.P. The experiment lasted for 60 days after induction of neurotoxicity. Food intake amount and rat's weight were recorded to obtain nutritional parameters. Blood samples were collected to assays the levels of Lactic Dehydrogenase (LDH), C- reactive protein level (CRP), Cyclooxygenase-2 (COX-2), Dopamine (DA), Serotonin (ST), Acetyl cholinesterase (AChE), some serum Heavy metals, Lipid peroxidation, Antioxidant enzymes activity, some kidney and liver functions. Also, the histopathological examination changes in brain tissue. The study results treated with CaNa2EDTA and ALA combined and ChS.P showed a significant improvement in serum levels of LDH, CRP, COX-2, DA, ST, AChE some Heavy metals, Lipid peroxidation, Antioxidant enzymes activity, and some kidney and liver function, when compared to untreated neurotoxicity group (+ve).
It can be recommend that the consumption of Alpha-lipoic acid and chia seed in diets have ant-neurotoxicity and antioxidants effects and that play significant safer role from calcium disodium edetate in treatment of lead-cadmium toxicity and inhibit of complications.