Pathogenic resistance to antibiotics, used for treating diseases, became an increased phenomenon with increasing pathogens. This study was conducted on real samples taken from cases admitted to Urology department at El-Mansoura hospital and did not normally respond to treatments by regular used antibiotics. The primarily samples investigation concluded (642) urine samples collected from patients suffered from urinary tract infections (UTI), 458 samples showed pathogenic bacterial growth on nutrient agar, while 184 samples showed no growth and considered free from UTI pathogens. The Gram reaction for picked colonies belonging to these levels revealed that 170 isolated were G^-ve, resistant to 2 types of out of 12 used antibiotics. 19 cases were G^+ve, resistant to 3 types. severe infection with number of pus less than 500 cells per field were represented by 98 cases. The pathogenic bacteria isolated from these cases were 91 G^-ve and 7 were G^-ve, resp.