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281877

Effect of Aerobic Fungal Inoculation on Silage Quality

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Last updated: 04 Jan 2025

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Abstract

This study sought to study the effect of certain strains of fungi (Trichoderma harzianum) and yeast (Saccharomyces cerevisiae D-47) inoculation on silage chemical composition, fermentation characteristics and in vitro digestibility.  Four treatments were tested i.e., control (C): corn silage without inoculants, Y: corn silage applied with saccharomyces cerevisiae D-47, T: corn silage applied with Trichoderma harzianum and Y+T: corn silage applied with both inoculants. Chopped whole corn were moved and sealed in polyethylene silo bags (1.5 to 2 kg) using a vacuum sealer for air removal. The silo bags were stored at room temperature for different ensiling times (zero time, 5 h, 10 h, 20 h, and 2, 4, 8, 14, 25 and 35 days). Obtained results showed that inoculants had no significant effect on DM and OM, while ensiling time significantly (P<0.04) decreased DM and OM content from 32.78% and 94.12% to 31.91% and 93.23% at 35d of ensiling respectively. treatments and ensiling time significantly (P< 0.5) increased CP and NFE in corn silage than the control (c) group, while CF, NDF and ADF significantly decreased, along with time of ensiling. Values of pH along with time of ensiling gradually decreased from 6.01 at zero time to 3.9 at 35d. Ammonia-N decreased significantly (P<0.001) from 39.06 in control comparing to 37.95, 38.44 and 37.0 g/kg total N. Ammonia nitrogen gradually increased significant (P<0.001) with the time of ensiling in a curve-linear way. Treating silages with inoculant yeast (Y), trichoderma (T) or both (Y+T) led to an increase (P<0.001) in lactic acid concentration being 39.5 and 38.99 g/kg DM, respectively. The best value was that of Y+T treatment (40.77g/kg DM), acetic and butyric acid followed opposite trend. Time of ensiling negatively correlated with concentration of both formic and citric acids while it was positively correlated with the concentration of succinic acids. Treating silages with inoculant increased total bacteria (5.51, 7.69, 7.69 and 7.81 log10 cfu/g DM) for the control, Y, T and Y+T, respectively. Similarly treating silages with inoculant significantly increased lactic acid bacteria (6.46, 6.89, 6.97 and 7.03 log10 cfu/g DM) for the control, Y, T and Y+T, respectively. Results of total yeasts (log10 cfu/g DM) followed the same pattern being less for control (5.45) and increased with the inoculant treatment being 6.57, 6.44 and 6.89 for the same respective order. Inoculants increased both IVDMD and IVOMD significantly; the best values were that of corn silage inoculated with both yeast + Trichoderma.

DOI

10.21608/mjapfp.2022.281877

Keywords

corn silage, inoculants, Saccharomyces cerevisiae D-47, Trichoderma harzianum, fermentation characteristics in- vitro digestibility

Authors

First Name

G. A.

Last Name

Baraghit

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Affiliation

Fac. of Agric., Menoufia Univ.

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Orcid

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First Name

B. M.

Last Name

Ahmed

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Affiliation

Fac. of Agric., Menoufia Univ

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City

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Orcid

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First Name

U. A.

Last Name

Nayel

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Affiliation

Fac. of Agric., Menoufia Univ

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Volume

6

Article Issue

11

Related Issue

39081

Issue Date

2022-12-01

Receive Date

2023-01-24

Publish Date

2022-12-01

Page Start

189

Page End

190

Print ISSN

2357-0814

Online ISSN

2735-3478

Link

https://mjapfp.journals.ekb.eg/article_281877.html

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https://mjapfp.journals.ekb.eg/service?article_code=281877

Order

3

Type

Original Article

Type Code

1,384

Publication Type

Journal

Publication Title

Menoufia Journal of Animal Poultry and Fish Production

Publication Link

https://mjapfp.journals.ekb.eg/

MainTitle

Effect of Aerobic Fungal Inoculation on Silage Quality

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Article

Created At

26 Dec 2024