Fenugreek (FG) flour is a common plant used in Egypt to add flavour and colour to food, keep food fresh, and as a medicine. The current study tested FG for its antioxidant, antidiabetic, antimicrobial and anticancer activities. FG showed significant contents of phenolic and flavonoid compounds, reflecting their nutraceutical behaviors. The EC₅₀ of FG when tested for its DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging was 2476 ± 62.9 µg/mL, while the FG extract's concentration that inhabited ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) was 37.13 ± 1.24 µg/mL. FG could lower blood sugar, as it showed an alpha-glucosidase inhibiton at EC₅₀ > 1000 µg/mL. Almost 99% of breast cancer cells were damaged by FG extract concentrations > 100 μg/mL. FG extract was successful in antimicrobial activity against Escherichia coli ATCC 8739 at a 1000 µg/mL concentration. The current study showed the promising usage of FG as a functional additive to different foods.