Background: Salmonellosis is a significant food-borne zoonosis and public health issue worldwide. Retail meat is considered as one of the major sources of Salmonella. However, data about the prevalence of Salmonella from mutton meat in Sudan are insufficient. Methods: This cross sectional study was undertaken to determine the prevalence and antimicrobial resistance of Salmonella spp. from mutton meat carcasses at butcher shops in Khartoum State, Sudan between February-December 2021. Results: Overall, Salmonella spp. was isolated from 6 samples out of 500 (1.2%).  All recovered isolates were sensitive to amikacin, cefotaxime and co-trimoxazole (100%), and 4 (66.6%) were resistant to ampicillin, 1 (16.6%) to cefuroxime and 1 (16.6%) to tetracycline.  None of the isolates showed multiple drug resistance (MDR).Conclusions: Our findings showed low prevalence of Salmonella in mutton meat in Khartoum State, and exhibited resistance to some antimicrobial agents. However, control measures should not be neglected to avoid contamination with food-borne pathogens.