Beta
335945

Controlling foodborne pathogenic Citrobacter freundii via lytic bacteriophages

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

-

Abstract

Recently, foodborne diseases caused by pathogenic bacteria have been expanding. This requires searching for ways to control or get rid of these diseases. Using bacteriophages to control, prevent, and treatment of foodborne diseases is one of the most important actions due to their killer effect on bacteria. Many species of pathogenic bacteria can be transmitted through food, including Citrobacter freundii which can cause food poisoning, urethritis, abscess, and meningitis in infants. This study aimed to isolate and characterize bacteriophages specific to Citrobacter freundii to be used to control the contamination of food with Citrobacter freundii. C. freundii and its specific bacteriophages were isolated from sewage water. Isolated phages were found to be belonging to two phage species. These two phages had a head and tail and were designated Citro 1 and Citro 2. Phage Citro 1 has a long contractile tail and Citro 2 has a long non-contractile tail. Therefore, they were classified into Family Myoviridae and Family Siphoviridae, respectively. Application of bacteriophages to green salad artificially contaminated with C. freundii resulted in a high reduction in the density of C. freundii in green salad kept at both room temperature and at 4°C. Moreover, when the density of C. freundii decreased the number of phage particles increased.  Such results may indicate that bacteriophages can be used to control the contamination of foodstuffs with pathogenic bacteria.  

DOI

10.21608/svuijas.2023.251917.1323

Keywords

Bacteriophages, Citrobacter freundii, Foodborne diseases, Pathogenic

Authors

First Name

Mazhar

Last Name

Mohamed

MiddleName

D.A.

Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Sohag University, 82524 Sohag, Egypt

Email

mazhareisawi@yahoo.com

City

-

Orcid

-

First Name

Mohamed A.

Last Name

Kelany

MiddleName

-

Affiliation

Food Science and Nutrition Department, Faculty of Agriculture, Sohag University, 82524 Sohag, Egypt

Email

-

City

-

Orcid

-

First Name

Adel

Last Name

Hammad

MiddleName

M.M.

Affiliation

Department of Microbiology, Faculty of Agriculture, Minia University, 61519 Minia, Egypt

Email

amhammad2000@yahoo.com

City

-

Orcid

-

First Name

Mohamed, H. M.

Last Name

Ahmed

MiddleName

-

Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Sohag University, 82524 Sohag, Egypt

Email

-

City

-

Orcid

-

First Name

Tarek

Last Name

Elsharouny

MiddleName

H.M.

Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Sohag University, 82524 Sohag, Egypt

Email

elsharouny@agr.sohag.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

4

Related Issue

39221

Issue Date

2023-10-01

Receive Date

2023-11-28

Publish Date

2023-12-31

Page Start

121

Page End

129

Print ISSN

2636-3801

Online ISSN

2636-381X

Link

https://svuijas.journals.ekb.eg/article_335945.html

Detail API

https://svuijas.journals.ekb.eg/service?article_code=335945

Order

335,945

Type

Original Article

Type Code

1,132

Publication Type

Journal

Publication Title

SVU-International Journal of Agricultural Sciences

Publication Link

https://svuijas.journals.ekb.eg/

MainTitle

Controlling foodborne pathogenic Citrobacter freundii via lytic bacteriophages

Details

Type

Article

Created At

25 Dec 2024