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327722

OPTIMIZATION OF KOJIC ACID PRODUCTION BY ASPERGILLUS COCULTURE AND IT’S APPLICATION AS ANTI-BROWNING AGENT

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Last updated: 25 Dec 2024

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Abstract

Kojic acid has applications in many fields so it is important to increase the amount of kojic acid. In this study production of kojic acid increased using coculture of Aspergillus oryzae ASU44 (OL314732) and A. flavus ASU45 (OL314748) than single culture, after optimizing by Box-Behnken statistical design, the production enhanced to 114.28 g/l using Glucose (150, g/l) (A), Yeast extract (5 g/l) (B), KH2PO4 (3 g/l) (C), MgSO4.7H2O (0.5 g/l) (D) and pH (3) (E). The design was effective and applicable with coefficient (R2) 99.1% and adjusted R2 value 98.1%. Ethyl acetate was the best extraction solvent with ratio 1:1 and the crystals of kojic acid appeared in needle shape after evaporation of ethyl acetate using rotatory evaporator. The crystalized kojic acid has the ability to inhibit the fungal pigment of A. niger ASU311. By increasing the KA concentration, the black color decreased, especially at 10 µg/ml comparing with the control samples. On the other hand, crystalized KA preserved the color of apple juice from the conversion to brown color and the activity increased by increasing the concentration until 50 µg/ml for 6 hrs comparing with the control. Application of kojic acid produced naturally using Aspergillus coculture considered safe and environmentally friendly for increasing the freshness of the fruit juice.

DOI

10.21608/bfsa.2023.327722

Keywords

Co, culture. Fungi. Experimental design. Decolorization

Authors

First Name

Nahla

Last Name

Kamal-Eldin

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Assiut University, P.O. 71516, Assiut, Egypt

Email

nahla.ahmed12331@gmail.com

City

-

Orcid

-

First Name

Abdel- Naser

Last Name

Zohri

MiddleName

A.

Affiliation

Botany and Microbiology Department, Faculty of Science, Assiut University, P.O. 71516, Assiut, Egypt

Email

zohriassiut@yahoo.com

City

-

Orcid

-

First Name

Ghada

Last Name

Mahmoud

MiddleName

Abd‐Elmonsef

Affiliation

Botany and Microbiology Department, Faculty of Science, Assiut University, P.O. 71516, Assiut, Egypt

Email

ghadamoukabel@aun.edu.eg

City

Assiut

Orcid

0000-0002-4760-9871

Volume

46

Article Issue

2

Related Issue

44483

Issue Date

2023-12-01

Receive Date

2023-06-07

Publish Date

2023-12-01

Page Start

1,269

Page End

1,283

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 2_Pages 1269-1283.pdf

PDF . 1.6MB

Link

https://bpsa.journals.ekb.eg/article_327722.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=327722

Order

327,722

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

OPTIMIZATION OF KOJIC ACID PRODUCTION BY ASPERGILLUS COCULTURE AND IT’S APPLICATION AS ANTI-BROWNING AGENT

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Type

Article

Created At

25 Dec 2024