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301135

IN- VITRO EVALUATION OF NANOCURCUMIN AGAINST MULTI-DRUG RESISTANT BACTERIA

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Last updated: 25 Dec 2024

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Abstract

A natural substance called curcumin, derived from the Curcuma longa plant, is frequently used to fight both gram-positive and gram-negative bacteria.It is poorly soluble in water so it has low bioavailability. The aim of this work was to develop effective nanocurcumin formulation to enhance its water solubility and antibacterial activity against four types of multidrug resistant strains. Nanocurcumin was formulated with different combined stabilizers employing antisolvent nanoprecipitation approach and then evaluated in terms of particle size, zetapotential and transmission electron microscopy. The optimized nanocurcumin formulation containing and Polyvinylpyrrolidone K30 (PVP) and Polyethylene glycol 6000(PEG6000) was selected for the In-vitro antibacterial studies against Staphylococcus aureus ATCC® 43300™*, Escherichia coli ATCC® 8739™*, Pseudomonas aeruginosa ATCC® 27853™* and Klebsiella pneumoniae ATCC® 33495™* compared with free curcumin. The prepared nanocurcumin formulation showed mean particle size of 272.15 ± 34.5 nm and polydispersity index (PDI) of 0.423 and high drug content percent ((95.5 ± 1.5 %).   The in-vitro antibacterial studies exhibited that the minimum inhibitory concentration (MICs) of free curcumin are 5011, 2511, 10000 and 10000 µg/ml against Staphylococcus aureus ATCC® 43300™*, Escherichia coli ATCC® 8739™*,  Pseudomonas aeruginosa ATCC® 27853™* and Klebsiella pneumoniae ATCC® 33495™*, respectively while the MICs of nanocurcumin are 128.8, 549.5, 67.60 and 312.2 µg/ml against Staphylococcus aureus ATCC® 43300™*, Escherichia coli ATCC® 8739™*,  Pseudomonas aeruginosa ATCC® 27853™* and Klebsiella pneumoniae ATCC® 33495™*,  respectively.  It showed highly reduced growth rate of tested strains. Curcumin in nanosized form has remarkable antibacterial activity against Staphylococcus aureus ATCC® 43300™*, Escherichia coli ATCC® 8739™*, Pseudomonas aeruginosa ATCC® 27853™* and Klebsiella pneumoniae ATCC® (MDRO) compared with free curcumin.

DOI

10.21608/bfsa.2023.301135

Keywords

Curcumin, Nanocurcumin, multidrug resistant organism

Authors

First Name

Noha

Last Name

Ibrahim

MiddleName

Mohammed Safwat

Affiliation

Department of Microbiology and Immunology, Faculty of Pharmacy, Assuit University

Email

nohasafwat26@gmail.com

City

assiut

Orcid

-

First Name

Ehsan

Last Name

B. Hassan

MiddleName

A.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt

Email

-

City

-

Orcid

-

First Name

Sherein

Last Name

Elgendy

MiddleName

G.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt

Email

shereinelgendy@aun.edu.eg

City

-

Orcid

-

First Name

Hebatallah

Last Name

Hassan

MiddleName

M.

Affiliation

Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Egypt

Email

-

City

-

Orcid

-

First Name

Abeer

Last Name

Hassan

MiddleName

S.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, South Valley University, Egypt

Email

-

City

-

Orcid

-

First Name

Niveen

Last Name

El-Gindy

MiddleName

G.

Affiliation

Department of Industrial Pharmacy, Faculty of Pharmacy, Assiut University, Egypt

Email

-

City

-

Orcid

-

Volume

46

Article Issue

1

Related Issue

41495

Issue Date

2023-06-01

Receive Date

2022-12-21

Publish Date

2023-06-01

Page Start

433

Page End

448

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 46_Issue 1_Pages 433-448.pdf

PDF . 1.6MB

Link

https://bpsa.journals.ekb.eg/article_301135.html

Detail API

https://bpsa.journals.ekb.eg/service?article_code=301135

Order

301,135

Type

Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

IN- VITRO EVALUATION OF NANOCURCUMIN AGAINST MULTI-DRUG RESISTANT BACTERIA

Details

Type

Article

Created At

25 Dec 2024