This study aimed to evaluate the Safety and wholesomenessof broiler feeds by identifying Aspergillus species, and detecting contamination with total aflatoxins in both starter (mashed) and finisher feed used to feed poultry (broilers). Samples were collected from six different farms in the Tartous Governorate - Syria, during the period from September 2023 to January 2024. The samples were grown in the laboratory on Potato Dextrose Agar (PDA) culture. Feed samples were analyzed for total aflatoxin content using a high-performance liquid chromatography (HPLC) device. Four species of Aspergillus fungi were identified in the studied feed samples: Aspergillus terreus (60%), followed by A. niger (37.1%), A. flavus (1.9%), and A. nidulans  (1%), as it was found that A. terreus was the most prominent species isolated from starter (mashed)  feed with a frequency of (96.6%), while A. niger was the most prominent species isolated from finished feed with a frequency of (78.7%).The results of determining total aflatoxins from the studied samples showed that three samples of starter (mashed)  feed (50%) and four samples of finished feed (66.7%) contained aflatoxins at an average of 0.7 µg/kg and 1.2 µg/kg in the starter (mashed) and finisher feed, respectively. This study concludes that the ready-made broiler feed used in the studied farms was contaminated with different types of Aspergillus spp. fungi, and with low levels of total aflatoxins, which poses a threat to the poultry industry due to toxins produced by some fungal species, due to their cumulative effect.