Seven varieties of Barley (Hordeum vulgare I.) Giza 123, 125, 126 as six rowed barley, Giza 127 and 128 as two rowed barley and naked barley were evaluated for their chemical composition, content of 13-glucan fraction, and fatty acids of barley oil. Unsaponifiable matter and Tocopherals were also determined. The data revealed that the total hydrolizable carbohydrates, crude protein, lipids, crude Hoer and ash ranged between (63.32-66.55, 9.76-15.75, 1.28-2.66, 1.3-4.8, 2.13-3.14)% respectively. The 13-glucan content ranged from 3.33 to 4.56. The most predominant fatty acids of Barley oil were palmitic C16: 0. (21.17%) stearic C18: 0 (12.12%) oleic Cl 8:1 (20_64%) Linoleic C18: 2 (48.38%) and Linolenic C18:3 (6.22%). The major hydrocarbons of unsaponifiable matter were C24 (21.08%) and C32 (10.72%) where as the major sterols were campsterol (9.04%) and cholestrol (4.73%).